Microsoft Word - Final Nano medician.docx

Department of Organic Chemistry, Faculty of Chemistry, University of Kashan, Kashan, 87317, I.R. Iran. Nanobiotechnology Research Center, Avicenna Research Institute, ACECR, 1936773493, Tehran, Iran. [email protected]. Abstract: In this study we have developed a simple, cheap, and rapid method for purification of 6×histidine-tagged recombinant proteins with high yields. The new immobilized metal ion affinity adsorbent was synthesized following co-precipitation method in order to synthesis of superparamagnetic manganes ferrit nanoparticles, and subsequently coated with 3-aminopropyltriethoxysilane (APTES) through a silanization reaction. Manganese ferrite nanoparticles afforded bidentate ligands as a result of reaction between isatoic anhydride and amino-functionalized MnFe2O4. The stable surface functionalized nanoparticles were further linked with Ni for purification of 6× His-tagged proteins. The nanoparticles were approximately 75 nm in size and were stable and had negligible non-specific binding for protein.